Equipment and research facilities

The Instituto de Biociências (IB) possesses an excelent infrastructure composed by laboratories well equipped for different research areas and field work equipment. The IB has laboratories fully equipped for: molecular biology techniques, biochemistry and physiology, including DNA sequencers (Sanger), HPLCs, ultracentrifuges, thermal cyclers, real time PCRs, image and microscopy techniques, including electron microscopy (MEV and MET), confocal,, fluorescence microscopes, micro-tomograph; equipment and culture chamber for the maintnance and growth of several groups of organisms and cell lines.

The IB has a Electron Microscopy Center, a cryogene room (equipped with -80ºC ultrafreezers), Herbarium (SPF, with 200.000 exsiccates; 4.000 samples in wood collection; 550 type materials), vivarium and a garden with several greenhouses and hundreds of botanical species cataloged.

For field work, IB has a advanced base in Pirassununga in a "Cerrado" area, a laboratory truck, several 4x4 vehicles and many field equipment for autonomous diving, traps for different organisms, among others.

 

Main equipment/facilities

CENTRAL

  • Cryogenic room (equipped with -80ºC Freezers)
  • Electron microscopy center with MEV and MET infrastructure
  • Micro-CT scanner skyscan
  • Botanical gardens and several greenhouses
  • Laboratory truck
        

BOTANY

  • Centrifuges, ultra-centrifuges, micro centrifuges (refrigerated or not)
  • Sample concentrator
  • High performance liquid chromatograph – HPLC Waters/Millipore
  • High performance liquid chromatograph – Hewllet Packard
  • High performance liquid chromatograph – Shimadzu
  • High performance liquid chromatograph – Agilent
  • Gas chromatographs – CG Hewllet Packard
  • Mass spectrum - Hewllet Packard
  • Fluorometers and spectrophotometers
  • Incubators with agitation
  • Lyophilizer
  • Epi-fluorescence microscopes, stereo microscopes
  • Microscopes coupled to digital cameras and interactive measurement module
  • Proteomics platform Amersham-GE
  • Real time PCR Systems
  • Rooms and chambers for seaweed culture, plant cells and plants
  • DNA sequencers Applied Biosystems 310, 3100, 3700
  • Thermo-hygrometric control system
  • Thermocyclers (with and without gradient)
  • Ultra-Freezers

ECOLOGY

  • Drying Ovens
  • Automated Glasshouse
  • Germination chambers
  • Portable gas exchange analysis system (IRGA)
  • Fluorescence analyzing system for chlorophyll
  • Laminar flow hood
  • UV-Visible Spectrophotometer
  • Precision scales
  • Centrifuge
  • Water deionizer system
  • Atomic absorption chromatograph
  • Portable spectroradiometer - UV - NIR
  • High performance magnifiers
  • High performance microscopes
  • Filtration system and production of high purity water

FISIOLOGY

  • High performance liquid chromatographo – HPLC Waters/Millipore
  • Epi-fluorescence microscopes, stereo microscopes
  • Microscopes coupled to digital cameras and interactive measurement module
  • Spectrophotometers
  • Spectrophotometers nano drop
  • Thermocyclers
  • Ultra-Freezers
  • Real time PCR systems
  • Fluorimeter
  • Incubators with shaking
  • Centrifuges, ultra-centrifuges, microcentrifuges (refrigerated or not)
  • Cryostat Leica
  • Confocal microscope Zeiss

GENETIC AND EVOLUTIONARY BIOLOGY

  • Electron Microscopy Center featuring: cryostat (Leica), Transmission Electron Microscope (Zeiss), Scanning Electron Microscope (Zeiss), metallizer (BAL-TEC), Sputter Coater (Blazers), Critical Point Dryer (Blazers), Confocal Microscope (Zeiss)
  • High pressure liquid chromatography (HPLC) Shimadzu
  • Low pressure liquid chromatography (FPLC) Amersha Pharmacia Biotech
  • Disulfide detector
  • Peroxide electrode
  • Spectrophotometers
  • DNA sequencer MegaBACE 1000
  • DNA sequencer 3730 Applied Biosystems
  • Cytometric flow system
  • Ultra-Freezers

ZOOLOGY

  • Dermestário for cleaning organic materials and assembly skeleton
  • Stereo microscopes (Leica EZ4 systems with digital image capture and 3D software installation Zeiss Discovery V8)
  • Molecular biology laboratory equipped with centrifuges, and Thermoblocks baths, thermal cyclers, Nanodrop, electrophoresis systems and photodocumentation laminar flow. SpeedVac, oven, ice machine, autoclave, and other equipment.
  • Stainless steel tables and laboratories with facilities for dissection
  • Microscopes (Zeiss AXIOSCOPE; Zeiss axiophot2; Zeiss Axio Imager M2; Zeiss phase contrast; Olympus BX51-DCI-III). Equipment allows research techniques such as Brightfield, Phase Contrast, Interference Contrast, Polarization with the possibility of fluorescence
  • Rotative microtome (LEICA RM 2255 serie 5418)
  • Collection vehicles, including 4x4